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Proteins were fractionated electrophoretically on NuPAGETM 412% Bis-Tris Polyacrylamide Gel (Invitrogen) and then transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot Dry Blotting System (Invitrogen)

10.1002/jcb.240320105 440 NeurathH

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